Plant Tissue Culture Ppt Pdf _top_ -

: Gradually reduce humidity and increase light exposure to harden the plants for field survival. 5. Major Types of Tissue Culture Techniques Explant Used Primary Application Callus Culture Any differentiated tissue

Plant tissue culture, or in vitro culture, is a collection of techniques used to maintain or grow plant cells, tissues, or organs under sterile conditions on a nutrient culture medium of known composition. The Principle of Totipotency

Before entering the lab, the mother plant must be carefully selected. It should be healthy, vigorous, and true-to-type (genetically uniform). Stage 1: Initiation (Establishment of Aseptic Culture)

Toshio Murashige and Folke Skoog developed Murashige and Skoog (MS) medium, which remains the industry standard today. Basic Principles and Requirements plant tissue culture ppt pdf

History and Milestones (MS Medium development).

Plant Growth Regulators (The Auxin-Cytokinin balance matrix).

Genetic manipulation, such as genetic engineering (transformation) and soma-clonal variation selection. : Gradually reduce humidity and increase light exposure

Depending on the starting material (explant) and the objective of the study, tissue culture is classified into several distinct types: 1. Callus Culture

: Genetic mutations can occur due to long-term exposure to artificial PGRs.

(e.g., IAA, NAA, 2,4-D): Promote root development and callus formation. The Principle of Totipotency Before entering the lab,

Introduction & Concept of Totipotency (Use a graphic showing a single cell evolving into a tree).

An unorganized, proliferating mass of actively dividing, undifferentiated parenchyma cells.

Once sufficient shoots are formed, they are transferred to a medium enriched with auxins to induce strong root systems. Stage 4: Acclimatization (Hardening)